DMPK | Issue | Recommendation |
Solubility | Insoluable | Simulated fluid replace PBS buffer |
Permeability | Poor recovery | Addition of BSA,FBS |
Transporter | Substrate, or inhibitor | Specific transporter, hMDR1 Knockin MDCKll Cells Or, hBCRP Knockin MDCKll Cells |
Bioanalysis | Unstable | Deactivate enzyme in plasma or blood immediately |
Bioanalysis | Non specific binding | Detergent |
Bioanalysis | Fragment inLs-MS ion source | Optimized MS condition |
Metabolism | Diverse metabolism | Comprehensive metabolism system: microsome, hepatocyte, S9,cytosol;AO,UGT activity; CYP phenotyping |
PPB | Unstable | Enzyme inhibitor |
In vivo PK | Low bioavailability | No fasting |
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